Agronomy, Vol. 15, Pages 802: Genome-Wide Identification and Expression Analysis of the Pepper β-1,3-Glucanase Gene Family in Response to Phytophthora capsici Stresses

Agronomy doi: 10.3390/agronomy15040802

Authors:
Han Wang
Dongchen Li
Yu Zhang
Yanping Wang
Tingting Song
Congsheng Yan
Li Jia
Haikun Jiang

Peppers are widely cultivated around the world, yet they suffer from infections caused by Phytophthora capsici fungi throughout the year, which severely impacts their yield. The β-1,3-glucanase gene has been shown in previous studies to significantly enhance plants’ ability to cope with both biotic and abiotic stresses, including fungal infections. However, its function in peppers has not been reported. In this study, 80 CaBG genes were initially identified, before being filtered down to 54 CaBGs in peppers, and analyses conducted on the physicochemical properties, chromosomal localization, phylogenetic tree relationships, synteny, promoters, and gene expression levels of the BG gene family. The results indicated that these 54 CaBG genes are located on 12 chromosomes. Phylogenetic tree analysis classified the CaBGs into three subfamilies, α, β, and γ, each with its own specific functions, with the γ subfamily being associated with disease resistance in peppers. Synteny analysis showed that CaBG genes are relatively conserved and have not undergone extensive whole-genome duplication events. Promoter analysis indicated that CaBGs are induced by plant hormones and various external stressors. Transcriptomic and RT-qPCR analyses revealed that the CaBG7 and CaBG12 genes were significantly activated following infection by P. capsici, with the expression levels of these two genes being markedly higher in resistant plants compared to susceptible ones. Based on the phylogenetic tree and gene expression analysis, we propose that CaBG7 and CaBG12 may be key genes for disease resistance in chili peppers. This study provides a theoretical basis for breeding P. capsici-resistant chili peppers by molecular breeding methods.

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Han Wang www.mdpi.com