Biosensors, Vol. 15, Pages 500: Target-Guided Droplet Routing on MEDA Biochips Considering Shape-Dependent Velocity Models and Droplet Splitting

Biosensors doi: 10.3390/bios15080500

Authors:
Yuta Hamachiyo
Chiharu Shiro
Hiroki Nishikawa
Hiroyuki Tomiyama
Shigeru Yamashita

In recent years, digital microfluidic biochips (DMFBs), based on microfluidic technology, have attracted attention as compact and flexible experimental devices. DMFBs are widely applied in biochemistry and medical fields, including point-of-care clinical diagnostics and PCR testing. Among them, micro electrode dot array (MEDA) biochips, composed of numerous microelectrodes, have overcome the limitations of conventional chips by enabling finer droplet manipulation and real-time sensing, thus significantly improving experimental efficiency. While various studies have been conducted to enhance the utilization of MEDA biochips, few have considered the shape-dependent velocity characteristics of droplets in routing. Moreover, methods that do take such characteristics into account often face significant challenges in solving time. This study proposes a fast droplet routing method for MEDA biochips that incorporates shape-dependent velocity characteristics by utilizing the distance information to the target cell. The experimental results demonstrate that the proposed method achieves approximately a 67.5% reduction in solving time compared to existing methods, without compromising solution quality.

Source link

Yuta Hamachiyo www.mdpi.com