Chemosensors, Vol. 13, Pages 170: Chlorpyrifos Detection Based on 9-Fluorenone Oxime

Chemosensors doi: 10.3390/chemosensors13050170

Authors:
Edoardo Donà
Aleksandra Lobnik

Chlorpyrifos is one of the most toxic organophosphate pesticides, prompting its ban in Europe in 2020. Consequently, developing a detection method that is both selective and sensitive is essential for protecting human health and the environment. In this study, we report for the first time a fluorescent probe based on an oxime for the direct detection of chlorpyrifos. 9-fluorenone oxime, upon deprotonation with a phosphazene base, undergoes a nucleophilic attack on chlorpyrifos, resulting in a significant alteration of its fluorescence properties. Following careful optimization, the method demonstrated excellent linearity (R2 = 0.98) over a concentration range of 350 to 6980 μg/L, with a limit of detection of 15.5 μg/L. Furthermore, the probe was successfully applied to chlorpyrifos detection in water samples, yielding satisfactory results. This approach effectively overcomes the stability limitations of enzyme-based fluorescent sensors, offering a robust and innovative solution for the detection of organophosphate pesticides.

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Edoardo Donà www.mdpi.com