IJMS, Vol. 26, Pages 9260: Is dUTPase Enzymatic Activity Truly Essential for Viability?

International Journal of Molecular Sciences doi: 10.3390/ijms26199260

Authors:
Anatoly Glukhov
Ulyana Dzhus
Ilya Kolyadenko
Georgii Selikhanov
Azat Gabdulkhakov

The study of protein enzymatic activities has always been a significant area of scientific and industrial research. The key steps typically undertaken in the characterization of a certain enzyme family include establishing the mechanism of catalysis, measuring kinetic parameters, determining structural organization and the architecture of the catalytic center, and subsequent classification. In this review, we tried to touch upon only a few points from the classical description of enzymes of the dUTPase family and added some additional functional properties of a number of representatives of this family. The existence of such extra functions raises questions about the reasons for this function duality. Based on the information known in the literature and our previous research, in this review, we conclude that the enzymatic activity of dUTPases supplements other functions independent of the hydrolysis reaction occurring in the catalytic center. In this context, it seems that dUTP acts not just as a substrate but as a signaling molecule, whose binding induces the realization of a special, non-enzymatic role of dUTPases.

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Anatoly Glukhov www.mdpi.com